Characterization of a metallo-protease produced in solid state fermentation by a newly isolated Bacillus strain
A metalloprotease was characterised from a newly isolated Bacillus strain. The enzyme activity was stimulated by mono- and divalent metal ions at 5 and 10 mM concentration and inhibited by EDTA, DTT and B ME (95, 90 and 80%, respectively) at 10 mM concentration. The enzyme inhibition caused by 10 mM...
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Dokumentumtípus: | Cikk |
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2011
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Sorozat: | Acta biologica Szegediensis
55 No. 1 |
Kulcsszavak: | Természettudomány, Biológia |
Online Access: | http://acta.bibl.u-szeged.hu/23151 |
LEADER | 01522nab a2200205 i 4500 | ||
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008 | 161017s2011 hu o 0|| eng d | ||
022 | |a 1588-385X | ||
040 | |a SZTE Egyetemi Kiadványok Repozitórium |b hun | ||
041 | |a eng | ||
100 | 1 | |a Saxena Rajshree | |
245 | 1 | 0 | |a Characterization of a metallo-protease produced in solid state fermentation by a newly isolated Bacillus strain |h [elektronikus dokumentum] / |c Saxena Rajshree |
260 | |c 2011 | ||
300 | |a 13-18 | ||
490 | 0 | |a Acta biologica Szegediensis |v 55 No. 1 | |
520 | 3 | |a A metalloprotease was characterised from a newly isolated Bacillus strain. The enzyme activity was stimulated by mono- and divalent metal ions at 5 and 10 mM concentration and inhibited by EDTA, DTT and B ME (95, 90 and 80%, respectively) at 10 mM concentration. The enzyme inhibition caused by 10 mM EDTA was restored by 45-47% with 5 and 10 mM NaCl. These characteristics established the enzyme as a metalloprotease. Different agroindustrial substrates were used for economic production of the metalloprotease, among which rice bran was selected as best providing z1000 U protease /g of substrate in 72 h with 20% inoculum and 1:3 moisture content (w/v). The enzyme was thermostable (30-50oC) with maximum activity at 60°C. It also exhibited a broad pH activity range (6-9) with a maximum at pH 8. | |
695 | |a Természettudomány, Biológia | ||
700 | 0 | 1 | |a Singh Rajni |e aut |
856 | 4 | 0 | |u http://acta.bibl.u-szeged.hu/23151/1/5513.pdf |z Dokumentum-elérés |